Europe's longest 'rail motorway' service that transports unaccompanied trailers carrying goods from Spain to the UK via France has halted operations.
It comes after migrants at the "Jungle" camp near Calais repeatedly tried to break into them as they attempted to get to Britain, said port authorities.
The VIIA Britanica railway has now stopped its services until September and Calais port workers have staged a go-slow on a highway in protest.
Up to now, the rail motorway, which began operations in March, has allowed road hauliers to cross France in 22 hours, avoiding 745 miles of roads.
Services have been stopped over a "resurgence in migrant intrusions in past weeks," according to authorities.
Antoine Ravisse, who has been protesting against the Calais migrant crisis, said: "The situation keeps getting worse. We don't know what to do anymore to be heard.
"The migrants, some of whom are armed, climb into the trailers, they rip the tarpaulins.
"Our clients lose confidence in us, sometimes their merchandise is destroyed. The cost is enormous."
Trains on the VIIA Britanica have transported trailers from Calais to Le Boulou (at the border between France and Spain) six days a week, with one round trip per day.
At Calais, the trailers can remain unaccompanied as they are loaded onto ferries to cross the English Channel.
The rail motorway terminal in Calais is the first such terminal in a port.
Each train is 680m long and is made up of 20 cars, with enough capacity for 40 trailers.
The service was predicted to shift 40,000 trailers a year from the roads to the rails over the next five years
Around 4,500 migrants, mainly from Afghanistan and Sudan, are believed to live in the Jungle camp.